Analysis of Multiple Infection in Ixodic Ticks <i>Dermacentor reticulatus</i> in a Combined Natural Focus of Vector-Borne Infections in the Tomsk Region

The aim of the study was to characterize mono- and mixed infection with causative agents vector-borne infections in Dermacentor reticulatus ticks inhabiting one anthropourgic foci Tomsk Region. Materials methods . D. were collected from vegetation “on flag” forest park zones southern part August September, 2020–2021. To isolate nucleic acids homogenates mites subsequent PCR diagnostics, kits RealBest series (“Vector-Best”, Novosibirsk) used. 184 individual samples examined for presence molecular-genetic markers tick-borne encephalitis virus (TBEV), pathogenic borrelia, babesia, rickettsia, anaplasma, ehrlichia. Results discussion spectrum pathogens detected through assay anthropurgic Region 2020 2021 includes TBEV, babesia. highest rate has been established relation candidate rickettsioses ( Rickettsia sp.) babesioses Babesia sp.). overall frequency co-infection 12.88 %. Seven different associations infecting have identified. majority (88.24 %) cases rickettsia associations. Double registered 94 % total number variants. Markers four simultaneously species DNA, Borrelia burgdorferi s.l. DNA TBEV RNA) found sample. Changes areal against background an increase their numbers, determines need analyze epidemiological significance this combined infections, especially zone sympatry ixodic other species.